Antibiotic Sensitivity Pattern and Esbl Detection among Clinical Isolates of Escherichia Coli

Background: ESBL producing bacteria poses a major problem for clinical therapautics. The ESBL producing isolates of E.coli among other clinical isolates has been increasing over the past few years resulting in limitation of therapeutic options. Materials and methods: A total number of 20 clinical specimens of E.coli were obtained from different clinical samples. They were subjected for the antibiotic susceptibility pattern by Kirby bauer disc diffusion method. The 3 and 4 generation cephalosporins resistant isolates will be detected for ESBL production. Results: In our isolates, we have found increased percentage 14/20 (70%) of isolates showed sensitivity to amikacin followed by gentamicin, which showed sensitivity of 9/20 (45%). 80-90% of E.coli isolates showed resistance to cephalosporin group of drugs. All E. coli isolates 20/20 (100%) were found to be extended spectrum beta lactamase producers using Ceftazidime (30μg), ceftazidime/clavulanic acid (30/10μg) and 11/20 (55%) of isolates were shown to be positive for ESBL using cefotaxime (30μg), cefotaxime/clavulanic acid (30/10μg). Conclusion: The study reveals higher percentage of isolates were resistant to different drugs and ESBL producers were more. We conclude that resistant to cephalosporins were due to extended spectrum beta lactamase production in our isolates.